Species Identification
Species identification is performed using DNA markers, which are conserved
within a species but variable between species. This service provides
the identification of the species of origin from
evidence taken from illegal commercialization and poaching of game
animals.
For example, matching a DNA profile
from a sample taken from an illegal kill site to a sample
collected from a suspected poacher.
DNA sequencing of specific markers has
been established for the following:
- Examples of Wildlife & Fish Species
- Moose (Alces alces)
- White-tailed Deer (Odocoileus virginianus)
- Caribou (Rangifer tarandus)
- Red Deer (Cervus elaphus)
- Canadian Elk (Cervus canadiensis)
- Black Bear (Ursus americanus)
- Wild Turkey (Meleagris gallopavo)
- Harlequin Ducks (Histrionicus histrionicus)
- Common Eider (Somateria sp.)
- Mallard Duck (Anas pl)
- Atlantic Puffin (Fraterculini arctica arctica)
- Black Guillimot (Cepphus grille arcticus)
- Dovekie (Alle alle alle)
- Peregrine Falcons (Falco perigrinus)
- Gyrfalcon (Falco rusticolus)
- Walleye (Stizostedion vutreum)
- Caviar sp.
Examples of Domestic Species:
- Cattle (Bos domesticus)
- Lamb (Ovis aries)
- Pig (Sus domesticus)
- Canine (Canis sp.)

Population Identification
A Potential Use for Population Identification
in Ontario.
Population identification may prove useful in the prosecution of
those poaching Ontario moose and deer. For example, identifying
a moose that originated from northwestern Ontario that was claimed
as originating from northeastern Ontario.
At present, samples are being collected for Trent
University's Wildlife Forensic Laboratory to establish
a database for use in such analysis. Databases for the population
identification of tree species and various fish species are under
development.

Individual Identification
Individual identification is performed using individual specific
DNA profiles or "DNA Fingerprints". Microsatellite DNA
profiling has shown that tissue samples from various organs of an
animal show identical fingerprint patterns. These patterns have
been shown to vary between different animals of the same species.
For example, a DNA
profile can be used to match a sample taken from an illegal kill
site (gut pile)
to a sample seized from as suspected poacher (frozen meat). In addition
the analysis can determine
the number of animals involved in a fish or wildlife infraction.

Parentage Analysis
Parentage analysis can be performed using individual specific DNA
profiles or "DNA Fingerprints" to determine if captive
bred animals are the legal offspring of registered breeding pairs
or if animals have been illegally obtained from wild stocks. For
example, the analysis of Peregrine falcon and eider duck chicks
from breeding programs.
Identification of Mixed Game Animal Tissue
with Domestic Animal Tissue
Southern blot analysis of species-specific highly repetitive DNA
probes can be used to detect the presence of game animal in processed
meat samples, which include domestic animal tissue.
If you are interested in quantification of game in
a product, please contact us in advance. For some species the analysis
is still in the research and developement stages.
Sex Identification
Ungulates: Polymerase Chain Reaction (PCR) amplification
of X and Y chromosome-specific DNA allows the determination of the
sex of ungulates, including moose, white-tailed deer, caribou and
elk, lacking gender-specific morphological characteristics. This
process allows one to determine the sex
of an animal based on a blood or tissue sample.
Birds: Polymerase Chain Reaction
(PCR) amplification of Z and W chromosome-specific CDH gene allows
the determination of the sex of a number of bird species, including
falcons, common eiders, turkey, ovenbirds and more. This process
allows one to determine the sex of bird species based on
a blood, tissue or feathers obtained. Bird sexing is currently under
development.
Expert Testimony and Consultation
Researchers from the Wildlife Forensic DNA Laboratory provide expert
testimony and consultation services for Conservation officers. Dr.
Bradley N. White, Dina
Abdelhakim and Dr. Paul J. Wilson have provided testimony for
more that 30 cases over the last 12 years in the Ontario, Newfoundland
and Nova Scotia Provincial Courts.
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